Journal: International Journal of Molecular Sciences

Article Title: Role of Clostridium perfringens Enterotoxin on YAP Activation in Colonic Sessile Serrated Adenoma/Polyps with Dysplasia

doi: 10.3390/ijms21113840

Figure Lengend Snippet: Effect of CPE on EMT phenotype in HT29 and HCM116 human colon cancer cells. ( A ) Effect of CPE on cell growth in the two cell lines. ( B , C ) Effect of CPE pretreatment on cell growth. HT29 cells ( B ) and HCT116 cells were treated with CPE of IC5 for 24 h. ( C ). ( D ) Effect of CPE on cell invasion. ( E ) Effect of CPE on protein levels of factors associated with BRAF, Hippo pathway, and CLDN4. ( F ) Effect of CPE on protein levels of factors associated with EMT and stemness. ( G ) BRAF mutation, nuclear YAP and protein levels of factors associated with EMT were examined in 4 CRC cases with or without CPE expression. Error bar, standard deviation from 3 independent trials. CLDN4 M, membranous CLDN4; CLDN4 C, cytosolic CLDN4; EMT, epithelial-mesenchymal-transition; IC, inhibitory concentration.

Article Snippet: HT29 and HCT116 human OSCC cell lines were purchased from Dainihon Pharmaceutical Co. (Tokyo, Japan).

Techniques: Mutagenesis, Expressing, Standard Deviation, Concentration Assay

Journal: Scientific Reports

Article Title: Reconstructing temporal and spatial dynamics from single-cell pseudotime using prior knowledge of real scale cell densities

doi: 10.1038/s41598-020-60400-z

Figure Lengend Snippet: MAPiT derived Ki-67 distribution in an 11-day-old HCT116 cell spheroid. ( a ) Representative spheroid cross-section stained for Ki-67. Rectangles display cross sectional signal intensities for comparison with MAPiT. ( b ) Transversal quantified Ki-67 intensities in spheroid sections (rectangles in ( a ), \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$n=9$$\end{document} n = 9 ). ( c ) MAPiT employs cell density in radial-symmetric spheroids with radius \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$r$$\end{document} r , of which the derivation is contained in detail in the Materials and Methods. ( d ) Marginal Ki-67 signal density obtained by MAPiT from flow cytometric data (blue) closely match Ki-67 intensity profiles determined microscopically in spheroid cross-sections.

Article Snippet: The human colon carcinoma cell line HCT116 was obtained from the Banca Biologica e Cell Factory of the IRCCS Azienda Ospedaliera Universitaria San Martino in Genoa (ICLC HTL95025).

Techniques: Derivative Assay, Staining, Comparison

Journal: Scientific Reports

Article Title: Reconstructing temporal and spatial dynamics from single-cell pseudotime using prior knowledge of real scale cell densities

doi: 10.1038/s41598-020-60400-z

Figure Lengend Snippet: Studying cellular composition in spheroids of HCT116 cells with MAPiT. ( a ) Marginal signal densities \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$p(y| x)$$\end{document} p ( y ∣ x ) of indicated markers related to the distance from the surface, as obtained by MAPiT. Signal frequencies at the outermost layer and at 150 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\mu m$$\end{document} μ m distance from the spheroid surface, as indicated by the dashed rectangles, are shown for an exemplary 11-day-old spheroid. ( b ) Comparison of single-cell positions in pseudotime, as obtained by Wanderlust or DPT algorithms. ( c ) MAPiT robustly reconstructs cell positions, irrespective of the pseudotime algorithm used. Ranges show \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$50 \% $$\end{document} 50 % confidence intervals of the signal intensities obtained from transforming Wanderlust and DPT pseudotime data. ( d ) Schematic of spheroid growth. ( e ) Spheroid growth follows a linear growth model. Microscopically obtained data shows spheroid radius over 15 days after seeding from \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$n=23$$\end{document} n = 23 spheroids in three independent replicates. ( f ) Median profiles for Ki-67, RNA and p27, as obtained by MAPiT, were conserved throughout spheroid sizes (median of \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$n=3$$\end{document} n = 3 spheroids, in three independent replicates). Notable deviations towards the ends of the profiles (dashed) and thus within the centre of the spheroids arise from inaccuracies due to the low number of cells available for analysis at these locations.

Article Snippet: The human colon carcinoma cell line HCT116 was obtained from the Banca Biologica e Cell Factory of the IRCCS Azienda Ospedaliera Universitaria San Martino in Genoa (ICLC HTL95025).

Techniques: Comparison

Journal: Frontiers in Oncology

Article Title: Synergistic effects of 5-fluorouracil in combination with salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in colorectal cancer

doi: 10.3389/fonc.2025.1558290

Figure Lengend Snippet: (A, B) The SW480 cell line is treated with SAL and 5-FU, respectively, to inhibit cell proliferation. (C, D) The HCT116 cell line is treated with SAL and 5-FU to inhibit cell proliferation, respectively. (E) The combination of 2.5 μM SAL and 5 μM 5-FU significantly inhibited the cell viability of SW480 cell line for 24 and 48 h (F) The combination of 2.5 μM SAL and 5 μM 5-FU significantly inhibited the cell viability of SW480 cell line for 24 and 48 h (G, H) Fa-CI diagrams of different combinations of SW480 and HCT116 cell lines are shown, respectively (CI < 1 means that the drug combination has a synergistic effect). (I, J) The SAL and 5-FU isobolograms of SW480 and HCT116 are shown, respectively (locating inside the triangle has a synergistic effect). Compared with the control group: * or ns, p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human CRC cell lines SW480 and HCT116 were purchased respectively from Applied Biological Materials Inc. (abm, Shanghai, China) and Haixing Biosciences Biotechnology (Jiangsu, China), supplemented with 10% fetal bovine serum (FBS, Procell, Wuhan, China) and 1% penicillin–streptomycin solution (P/S, 100×, Procell, Wuhan, China).

Techniques: Control

Journal: Frontiers in Oncology

Article Title: Synergistic effects of 5-fluorouracil in combination with salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in colorectal cancer

doi: 10.3389/fonc.2025.1558290

Figure Lengend Snippet: (A) EdU staining results of SW480 and HCT116 cell lines after treatment with different drugs for 20 h. (B, C) Statistical analysis of EdU staining results of SW480 and HCT116 cell lines after administration. (D) Cell cycle staining results of SW480 and HCT116 cell lines after treatment with different drugs for 20 h. (E, F) Statistical analysis of cell cycle staining results of SW480 and HCT116 cell lines after administration. Compared with the control group: ns, p < 0.05; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human CRC cell lines SW480 and HCT116 were purchased respectively from Applied Biological Materials Inc. (abm, Shanghai, China) and Haixing Biosciences Biotechnology (Jiangsu, China), supplemented with 10% fetal bovine serum (FBS, Procell, Wuhan, China) and 1% penicillin–streptomycin solution (P/S, 100×, Procell, Wuhan, China).

Techniques: Staining, Control

Journal: Frontiers in Oncology

Article Title: Synergistic effects of 5-fluorouracil in combination with salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in colorectal cancer

doi: 10.3389/fonc.2025.1558290

Figure Lengend Snippet: (A) Annexin-V-FITC/PI apoptosis staining results of SW480 and HCT116 cell lines after treatment with different drugs for 20 h. (B, C) Statistical analysis of apoptosis staining results of SW480 and HCT116 cell lines after administration. (D) Crystal violet staining results of colony formation assay on HCT116 and SW480 cell lines after different treatments. (E, F) Statistical analysis of colony formation assay results of SW480 and HCT116 cell lines after administration. Compared with the control group: * or ns, p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human CRC cell lines SW480 and HCT116 were purchased respectively from Applied Biological Materials Inc. (abm, Shanghai, China) and Haixing Biosciences Biotechnology (Jiangsu, China), supplemented with 10% fetal bovine serum (FBS, Procell, Wuhan, China) and 1% penicillin–streptomycin solution (P/S, 100×, Procell, Wuhan, China).

Techniques: Staining, Colony Assay, Control

Journal: Frontiers in Oncology

Article Title: Synergistic effects of 5-fluorouracil in combination with salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in colorectal cancer

doi: 10.3389/fonc.2025.1558290

Figure Lengend Snippet: (A) Results of wound healing assays on HCT116 and SW480 cell lines after different administration treatments. (B, C) Statistical analysis of wound healing assays results of SW480 and HCT116 cell lines after administration. (D) Results of migration and invasion assays on HCT116 and SW480 cell lines after different administration treatments. (E–H) Statistical analysis of migration and invasion assay results of SW480 and HCT116 cell lines after administration, respectively. Compared with the control group: * or ns, p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human CRC cell lines SW480 and HCT116 were purchased respectively from Applied Biological Materials Inc. (abm, Shanghai, China) and Haixing Biosciences Biotechnology (Jiangsu, China), supplemented with 10% fetal bovine serum (FBS, Procell, Wuhan, China) and 1% penicillin–streptomycin solution (P/S, 100×, Procell, Wuhan, China).

Techniques: Migration, Invasion Assay, Control

Journal: Frontiers in Oncology

Article Title: Synergistic effects of 5-fluorouracil in combination with salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in colorectal cancer

doi: 10.3389/fonc.2025.1558290

Figure Lengend Snippet: (A) Transmission electron microscopy results of different group administration treatments. Among them, more autophagy lysosomes can be seen in both SAL and 5-FU. Ferroptotic mitochondrial changes (as shown by the green arrow) and a small number of autophagy lysosomes can be seen in the combination of the two drugs. (B) ROS staining results of SW480 and HCT116 cell lines after treatment with different drugs for 20 h (C, D) Statistical analysis of ROS staining results of SW480 and HCT116 cell lines after administration. (E, F) Intracellular MDA levels under different group drug treatments of SW480 and HCT116 cell lines. (G, H) Synergistically promote an increase in MDA levels, which can be restored by 100 mM NAC in SW480 and HCT116 cell lines. Compared with the control group: * or ns, p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human CRC cell lines SW480 and HCT116 were purchased respectively from Applied Biological Materials Inc. (abm, Shanghai, China) and Haixing Biosciences Biotechnology (Jiangsu, China), supplemented with 10% fetal bovine serum (FBS, Procell, Wuhan, China) and 1% penicillin–streptomycin solution (P/S, 100×, Procell, Wuhan, China).

Techniques: Transmission Assay, Electron Microscopy, Staining, Control

Journal: Frontiers in Oncology

Article Title: Synergistic effects of 5-fluorouracil in combination with salinomycin promoted ferroptosis via inhibiting SLC7A11/GPX4 in colorectal cancer

doi: 10.3389/fonc.2025.1558290

Figure Lengend Snippet: (A) Western blot shown the changes in expression levels of key ferroptosis proteins GPX4 and SLC7A11 in SW480 and HCT116 cell lines with different drugs for 20 h (B–E) Statistical analysis of corresponding protein, GPX4 and SLC711, of changes of SW480 and HCT116 cell lines after administration. (F) The protein expression levels of GPX4 and SLC7A11 that were reduced during the double-drug synergy can be restored to normal levels by 100 mM NAC. (G–J) Statistical analysis of counterpart proteins of SW480 and HCT116 cell lines after administration of combination and NAC. Compared with the control group: * or ns, p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human CRC cell lines SW480 and HCT116 were purchased respectively from Applied Biological Materials Inc. (abm, Shanghai, China) and Haixing Biosciences Biotechnology (Jiangsu, China), supplemented with 10% fetal bovine serum (FBS, Procell, Wuhan, China) and 1% penicillin–streptomycin solution (P/S, 100×, Procell, Wuhan, China).

Techniques: Western Blot, Expressing, Control